DNA
Part:BBa_J100174
Designed by: Hannah Itell Group: Campbell M Lab (2014-07-25)
Strong Promoter + M6-R1 Ammeline Riboswitch + RFP
This has a seven mutations from the original M6 riboswitch sequence found in part J119332. Five of these mutations are part of the intentional design of the riboswitch, in order for it to be more effective than the original M6 riboswitch. As with part J119332, base 432 is mutated from an A (tyrosine) to a G (cysteine) in the RFP CDS. The two additional mutations are T20C in the promoter region and C852T in the RFP CDS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 742
Illegal AgeI site found at 854 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |